CruzDB: software for annotation of genomic intervals with UCSC genome-browser data

Motivation: The biological significance of genomic features is often context-dependent. Annotating a particular dataset with existing, external data can provide insight into function. Results: We present CruzDB, a fast and intuitive programmatic interface to the UCSC genome browser that facilitates integrative analyses of diverse local and remotely hosted datasets. We showcase the syntax of CruzDB using miRNA-binding sites as examples, and further demonstrate its utility with 3 biological discoveries. First, we find that while exons replicate early, introns tend to replicate late, suggesting a complex replication pattern in gene regions. Second, variants associated with cognitive functions map to lincRNA transcripts of relevant function. Third, lamina-associated domains are highly enriched in olfaction-related genes. Availability: CruzDB is available at https://github.com/brentp/cruzdb Contact: [email protected],